Comparative proteome analysis of two antagonist Bacillus subtilis strains.

发布时间:2012-03-22 00:00:00 责任编辑:生物技术学院信息组 点击次数:246

Comparative proteome analysis of two antagonist Bacillus subtilis strains.

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Abstract: Natural wild-type strains of Bacillus subtilis are extensively used in agriculture as biocontrol agents for plants. This study examined two antagonist B. subtilis strains, KB-1111 and KB-1122, and the results illustrated that KB-1122 was a more potent inhibitor of the indicator pathogen than KB- 1111. Thus, to investigate the intrinsic differences between the two antagonist strains under normal culture conditions, samples of KB-1111 and KB-1122 were analyzed using MALDI-TOF-MS. The main differences were related to 20 abundant intracellular and 17 extracellular proteins. When searching the NCBI database, a number of the differentially expressed proteins were identified, including 11 cellular proteins and 10 secretory proteins. Among these proteins, class III stress-response-related ATPase, aconitate hydratase, alpha-amylase precursor, and a secretory protein, endo-1, 4-beta-glucanase, were differentially expressed by the two strains. These results are useful to comprehend the intrinsic differences between the antagonism of KB-1111 and KB-1122.

Zhang, C. X., X. Zhao, F. Han, M. F. Yang, H. Chen, T. Chida and S. H. Shen (2009). "Comparative proteome analysis of two antagonist Bacillus subtilis strains." J Microbiol Biotechnol 19(4): 351-357.

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